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Lesson 5: Chemical Analysis of Water
* A water
analysis lesson about water chemistry and water quality
Geared
toward 3rd through 6th grade (targeted 4th
grade)
Materials
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1 Low cost GREEN Water Monitoring kit from LaMotte per 2
students + 1 for demonstration
o
1 color card per 2 students plus one for demo
o
Test station set-ups for each test. Each test station includes
one of the GREEN Water Monitoring kit’s white plastic canister taped to a
stake and labeled with the test who’s supplies it will hold with laminated
copies of the test’s instructions, test tubes and tablets.
o
Nitrile gloves in various sizes (Latex gloves can set off
serious allergies), enough for all participants
o
Safety goggles for all participants
o
Sterilized bucket(s), 2- 2.5 gal with long rope tied to handle
to gather stream water, with a cap if transporting (can be sterilized by
boiling or cleaning and then rinsing with boiling water)
o
Waste water bucket
o
Trash receptacle for dropped tablets, aluminum foil wrappers
and used gloves
o
Waders or water proof boots for the person collecting water if
the water is to be used off site
o
One low cost GREEN water monitoring kit instruction booklet
Activities
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Set up supplies for the test, either placing what each student
team will need for all the tests in the supplied white, plastic canisters or
using the staked, labeled and pre-filled test station set-ups.
o
Discuss possible sources of impact on the streams and how they
might impact streams (people, tree coverage or lack there-of, animal
activity, etc.) and/or the needs of the fish and other living things in the
streams.
o
Demonstrate for the students the correct method for getting
the tablets out of the wrappers and into the test tubes (WSWCD staff can
demonstrate), reading the thermometers (pg. 28) and reading the secchi disk.
Make sure the students do the Dissolved oxygen first, unless you want them
to discover what happens if you wait, then have at least one student group
do them first and keep track of how long after the sample water was
collected from the stream each D.O. test was done.. Instruct them to shake
their water up before doing the Turbidity test (shaking will alter the
Dissolved Oxygen test). Stress that they do NOT shake the Coliform test tube
after adding water, just stand it upright.
o
Collect stream water
§
On-site testing – while maintaining grip on the rope, toss
bucket out upstream and pull it
back towards you, lift out of water before the bucket reaches shore, (WSWCD
staff can demonstrate)
§
Off-site testing (transporting water to class room to test)
refer to directions in the accompanying booklet, pg. 9.
o
Have students follow the easy to follow directions provided
for each test and match the resulting colors to the accompanying color
charts to find their test results (test tubes must be held up to the white
area to compare colors. If accurate D.O. readings are desired, do this test
first. Temperature should be done 2nd.
o
Students can grade their stream using the “ranking test
results” starting on page 32 of the manual or using a table we can
provide. They can do this for individual group results and/or class results.
If class and group results differ, they can discuss why they think that
happens. Have students discuss what the results mean and, if results were
not acceptable, what might be done to fix the problem?
o
Notes of special
interest –
§
As temperature increases, water’s ability to hold dissolved
oxygen decreases and organisms demand for oxygen increases.
§
As nitrates/phosphates increase, algae increases, potentially
blooming, so algae population is high enough to use up all the oxygen in the
water each night .
§
pH range for salmon and most other aquatic life is 6.5 to 8.5
§
Upper temperature limit for salmon is 13 oC for
spawning, egg and fry incubation and 15oC for salmon rearing and
migration
Mat-Su District 4th Grade Science Standards
addressed with this activity:
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A1.1 Asking questions, predicting, observing, describing,
measuring, making generalizations, inferring
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A1.1a Working collaboratively, carrying out investigations
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A1.1b Differentiating observation from inference and knowing
scientists’ explanations come partly from what they observe and how they
interpret their observations.
o
A1.1c Working with an increasing variety of models and tools.
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A1.2a Formulating and justifying predictions based on cause
and effect relationships
Time Required
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10 minutes to set-up stations
o
Per test:
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Dissolved oxygen (D.O.)
– 12 min. (5 just waiting)
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Temperature –
2 min.
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Turbidity –
2 min.
§
pH –
4 min.
§
coliform –
2 min. to mix, incubate 48 hrs
§
Nitrates –
9 min. (5 just waiting)
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Phophates
– 9 min. (5 just waiting)
§
BOD (pg. 21) –
5 day incubation, 12 min.
D.O. test
§
% saturation of dissolved oxygen – 14 min (12 min D.O.
test
+ 2 min. using chart on
page.19)
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20 minutes to clean up
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